Ko te huakita entomopathogenic, ko Xenorhabdus nematophila, ka whakaawe i te aukati i te mate o nga pepeke e whai ana ma te aukati i te mahi phospholipase A(2) (PLA(2)).Ina tata nei, i kitea he ira PLA(2) e pa ana ki te aukati mai i te namu paraoa whero, Tribolium castaneum.Ko te rangahau nei i whakakaohia tenei ira PLA(2) ki roto i te kaitao huakita huakita hei whakaputa i te whaariki whakahiato.Ko te T. castaneum PLA(2) (2) (TcPLA(2)) recombinant i whakaatu i tona ahuatanga o te mahi enzyme me te kukū o te tïpako, te pH, me te pāmahana ambient.Ko ona ahuatanga matū koiora he rite ki tetahi momo huna o te PLA(2) (sPLA(2)) na te mea i aukatihia tana mahi e te dithiothreitol (he kaihoko whakaheke i te herenga disulfide) me te bromophenacyl bromide (he aukati sPLA(2) motuhake) engari ehara i te methylarachidonyl. fluorophosphonate (he momo cytosolic motuhake o PLA(2)).Kei roto i te hupa ahurea X. nematophila he PLA(2) tauwehe aukati, he mea nui rawa atu i roto i te papapāho i riro mai i te wahanga tipu huakita.Ko te (2) tauwehe aukati i te wera, ka tangohia i roto i nga hautau wai me te hautau waro.Ko te paanga o te hautanga aukati PLA(2) i runga i te whakakorenga mate o te T. castaneum i rite ki tera i puta mai i te aukati i te whakaaturanga ira TcPLA(2) na te wawaotanga RNA.
