4-Amino-3-hydrazino-1,2,4-triazol-5-thiol Cas:28836-03-5 99% Kōwhai ki te mārō kākāriki
| Nama Putumōhio | XD90146 |
| Ingoa Hua | 4-Amino-3-hydrazino-1,2,4-triazol-5-thiol |
| CAS | 28836-03-5 |
| Tātai rāpoi | C16H13NO3S·NH3 |
| Taumaha rāpoi | 316.37 |
| Nga Taipitopito Rokiroki | Ambient |
| Waehere Rere Whakariterite | 2923900090 |
Whakatakotoranga Hua
| Te ahua | Kowhai ki te kakariki totoka |
| Ahahay | ≥ 99% |
| Te rewa | 237°C(dec.)(marama) |
| Wairewa | NaOH: wairewa1 N |
| Wairewa wai | Wairewa ki te wai, 1N NaOH, me te mewaro. |
1. I akohia e matou nga paanga aukati o te isorhamnetin ki te tyrosinase mushroom na roto i nga kinetics aukati me te whaihanga rorohiko.Ko te Isorhamnetin i whakakorea te tyrosinase i roto i te momo whakauru i Ki=0.235±0.013 mM.Ko nga inenga o te intrinsic me te 1-anilinonaphthalene-8-sulfonate (ANS) -binding fluorescence i whakaatu ko te isorhamnetin kaore i puta nga huringa nui i roto i te hanganga tuatoru o te tyrosinase.Kia mohio ai koe ki te mahi whakawetohanga, i tatauhia nga kinetika ma te inenga wa-waa me nga tauhohenga tïpako tonu.Ko nga hua i tohu ko te whakakore i te isorhamnetin he tauhohenga tuatahi me nga tukanga biphasic.Hei whai matauranga ake, i whakatairitehia e matou te tauranga i waenga i te tyrosinase me te isorhamnetin.I angitu te whaihanga (nga kaha here mo Dock6.3: -32.58 kcal/mol, mo AutoDock4.2: -5.66 kcal/mol, a mo Fred2.2: -48.86 kcal/mol), e kii ana ka uru te isorhamnetin ki etahi toenga, penei hei HIS244 me MET280.Ka whai hua pea tenei rautaki o te tauwhitiwhitinga o te tyrosinase ting te matapae me te kinetics i runga i te puhui flavanone ki te tirotiro mo nga aukati tyrosinase taiao.
2.Ko te ara whakakitenga waikawa o te conalbumin (CA), he glycoprotein monomeric mai i te heihei ma, kua tirotirohia ma te whakamahi i te hihinga CD tawhiti me te tata-UV CD, te tukunga rerewharewha o roto, te rangahau fluorescence o waho 1-anilino-8-napthalene sulfonate (ANS) me te marara marama hihiri (DLS).Ka kite matou i nga huringa e whakawhirinaki ana ki te pH i te hanganga tuarua me te hanganga tuatoru o CA.He rite tonu te ahua o te α-helicic hanganga tuarua ki te pH 4.0 engari te hanga ngaro ki te pH 3.0.Ko te CA anake te ahua o te ahua o te ao o mua-whakarewa me te ahua o te rewa i roto i te otinga i te pH 4.0 me te pH 3.0.Ko te paanga o te pH ki te ahua me te hauhautanga o te CA e tohu ana ki tona parenga wera i te pH koretake.Ko nga hua DLS e whakaatu ana he ahua totika te ahua o te MG i roto i nga wairewa wai me te radii hydrodynamic o te 4.7 nm.Ko te tineia o te tryptophan fluorescence na roto i te acrylamide i whakapumau ake i te kohi o te ahua takawaenga, kua wehea tetahi wahi, i waenga i nga whenua taketake me nga whenua kore.
![N-[[bis[4-(dimethylamino)phenyl]amino]carbonyl] glycine konutai tote Mā ki te hina-matomato paura tioata](http://cdn.globalso.com/xdbiochems/115871-19-7.jpg)
