4-Nitrophenyl-alpha-D-galactopyranoside CAS:7493-95-0 99%
| Nama Putumōhio | XD900018 |
| Ingoa Hua | 4-Nitrophenyl-alpha-D-galactopyranoside |
| CAS | 7493-95-0 |
| Tātai rāpoi | C12H15NO8 |
| Taumaha rāpoi | 301.25 |
| Nga Taipitopito Rokiroki | -15 ki te -20 °C |
| Waehere Rere Whakariterite | 29400000 |
Whakatakotoranga Hua
| Te ahua | Ma ki te Karaka ki te Kakariki paura ki te karaihe |
| Te whakamatautau | 99% |
Ko te 4-Nitrophenyl α-D-galactopyranoside (PNP-alpha-D-Gal) he taputapu artificial o 4-nitrophenyl (pNP) glycopyranoside mo te kitenga o te mahi α-galactosidase.Ko te nui o te pNP i tukuna i tino piki ake i te wa i whakamahia ai te 4-Nitrophenyl α-D-galactopyranoside hei taputapu.
Ma te whakamahi i te 4-nitorophenol (pNP) -glycopyranosidespseudosubstrates, te nui o te pNP kua wetekina mai i te 4-Nitrophenylα-D-galactopyranoside he mea tino nui ake i tera atu i tera atuNP-glycopyranosidesatpH4.0 engari ko te kore mahi i kitea i te pH07. pyranoside kaaahua te turanga kawakore e whakaatu ana i nga toenga o te galactose kua tukuna mai i te 4-Nitrophenylα-D-galactopyranoside i te nuinga o te pH4.0, i te taumataThei te hekenga o te paepae i te nuinga.
Te papanga o te α-D-galactosidase (α-D-Galactosidase).Ka herea ki te kawe pūmua E. coli lactose.Waenga mo te α-D-galactose.
Kua whakawhanakehia he tikanga tere mo te aromatawai i te mahi lytic o nga kaihoko antimicrobial ki te rewena me te harore.Ko te whakamatautau i runga i te tukunga o te enzyme intracellular, maltase (alpha-glucosidase).Ko te mahi maltase i tukuna i inehia ma te tae ma te hanga p-nitrophenol mai i te p-nitrophenyl-alpha-D-glucopyranoside (PNPG).I whangangahia te mahi lytic o nga momo momo momo momo puhui antimicrobial ki nga pūtau rewena me nga pua tipu o te harore kawau.Ka kitea te mahi anti-rewena lytic i roto i te 20 min te heki i te 30 nekehanga C ki a Saccharomyces cerevisiae, Candida albicans, me Cryptococcus neoformans.I puta mai te mahi anti-fungal Lytic i muri i te 2 h o te incubation i te 30 nga nekehanga C ki nga pua tipu o Aspergillus niger me Botrytis cinerea.Ko nga pūtau katoa o te rewena, te harore ranei, karekau i tino whakamaarama i te PNPG i roto i te 3 haora i te 30 nga nekehanga C kia puta he huringa tae ka kitea.Ko te mahi lytic o nga enzymes (hei tauira, Lyticase), paturopi (hei tauira, Amphotericin B), me tetahi riaka huakita hua paturopi i kitea ma te whakamahi i te whakamatautau.Kua urutauhia te whakamaarama anti- rewena ki te whakatakotoranga microtiter 96-puna.Ko nga whakamatautau e rua i whakarato i te kitenga tere, tairongo, me te whakahoki ano mo te mahi anti-weeke me te mahi anti-fungal.
