4-NITROPHENYL-ALPHA-D-MANNOPYRANOSIDE CAS:10357-27-4 Paura-Whio 98%
| Nama Putumōhio | XD90011 |
| Ingoa Hua | 4-Nitrophenyl-alpha-D-mannopyranoside |
| CAS | 10357-27-4 |
| Tātai rāpoi | C12H15NO8 |
| Taumaha rāpoi | 30301.25 |
| Nga Taipitopito Rokiroki | -2ki-8°C |
| Waehere Rere Whakariterite | 29400000 |
Whakatakotoranga Hua
| Wai | <5% Karl Fische |
| Wairewa | 1% i roto i te DMF he maamaa me te kore tae |
| Maamaa | Koreutu 4-Nitrophenol <200ppm |
| HPLC | >98% |
| Te ahua | Te paura ma |
Nga tirohanga miihini ki te whanau o te alpha-mannosidases e whakawhirinaki ana ki te Ca2+ i roto i te ahua o te kopu tangata.
Ko nga huakita koroni, e whakaatuhia ana e Bacteroides thetaiotaomicron, he mahi nui ki te pupuri i te hauora o te tangata ma te whakamahi i nga whanau nui o te glycoside hydrolases (GHs) ki te whakamahi i nga polysaccharides kai me nga glycans manaaki hei matūkai.Ko taua whakawhänui whänau GH kua whakatauirahia e te whänau 23 GH92 glycosidases kua whakawaeherehia e te B. thetaiotaomicron genome.I konei ka whakaatuhia e matou he alpha-mannosidases enei e mahi ana ma te tikanga whakanekehanga kotahi hei whakamahi i nga N-glycans kaihautu.Ko te hanganga e toru-ahu o nga GH92 mannosidases e rua e tautuhi ana i te whanau o nga pūmua e rua-rohe kei reira te pokapū catalytic kei te atanga rohe, e whakarato ana i te waikawa (glutamate) me te turanga (aspartate) awhina ki te hydrolysis i roto i te Ca(2+)- tikanga whakawhirinaki.Ko nga hanganga ahu-toru o nga GH92 i roto i te matatini me nga aukati e whakarato ana i te maaramatanga ki te motuhake, te tikanga me te huarahi whakarite o te catalysis.Ka whai waahi nui a Ca(2+) ki te whakakorikori i te mannoside mai i te ahua o te tuuru (4)C(1) ki te ahua whakawhiti.（Bibliography: Nat.Chem.Koiora.6, 125-32, (2010)
Te chromatography hononga o mua o te ovalbumin glycoasparagines i runga i te pou concanavalin A-sepharose.He rangahau ine mo te herenga motuhake o te lectin.
Ko nga taunekeneke a Sepharose 4B-immobilized concanavalin A (ConA) me te 10 glycoasparagines i ahu mai i te ovalbumin i tirotirohia i te rahi e te chromatography affinity o mua.I roto i tenei tikanga, ka tukuna tonutia he otinga warowaiha ki tetahi pou ConA-Sepharose ka inehia te whakamuri o te mua elution hei tawhā o te kaha o te taunekeneke.Ka taea te whakatau i te tauwehenga (Kd) mo ia saccharide me te ConA.Ko te tātaritanga o te herenga o te p-nitrophenyl-alpha,D-mannoside i whakaatu ko nga ahuatanga here o ConA kaore e tino rerekee i muri i te korenga i runga i te Sepharose 4B.Ko ia ovalbumin glycoasparagines i tapaina ki te tritium e te tikanga methylation whakaheke mo te tātari.Ko te whakatauritenga o nga uara Kd i kitea he rerekee te herenga o te ConA me te iti rawa o nga rereketanga hanganga o te mekameka glycosyl.Ko nga hua e whakaatu ana ka mohio a ConA ki tetahi hanganga mekameka glycosyl motuhake, Man alpha 1-6(Man alpha 1-3)Man, i roto i te iti rawa kia kotahi te roopu hydroxyl i te waahi C-3 o te mannose hono-C-6 me kore utu.
