Ko te biosynthesis o te proteoglycans me te glycosaminoglycans i te aroaro o te p-nitrophenyl-xyloside i akohia ma te whakamahi i te punaha ahurea o te kiore ovarian granulosa tuatahi.Ko te taapiri o te p-nitrophenyl-xyloside ki roto i te reo ahurea pūtau ka nui ake te 700% o te whakaurunga o te [35S]sulfate (ED50 i te 0.03 mM) ki roto i nga macromolecules, kei roto ko nga mekameka chondroitin sulfate koreutu i timatahia i runga i te xyloside me te proteoglycans taketake.Ko nga mekameka chondroitin sulfate koreutu i timatahia i runga i te xyloside he mea huna noa ki roto i te reo.Ko te rahi o te ngota ngota o nga mekameka chondroitin sulfate i heke mai i te 40,000 ki te 21,000 i te mea kua whakanuia te katoa o te [35S] kaporeihana sulfate, e kii ana ko te whakahiatotanga o te chondroitin sulfate i whakararu i te tikanga o te mutunga o te mekameka glycosaminoglycan.Ko te biosynthesis o te heparan sulfate proteoglycans i heke tata ki te 50%, na te whakataetae i te taumata o te UDP-huka mua.[35S]I tutakina te kaporeihana ngawha ma te taapiri o te cycloheximide me te hawhe wa tuatahi tata ki te 2 haora i te aroaro o te xyloside, i te mea kaore he xyloside he 20 meneti pea.Ko te rereketanga pea e whakaatu ana i te tere hurihanga o te kaha whakahiato glycosaminoglycan i te katoa.Ko te tere o te hurihanga o te kaha whakahiato glycosaminoglycan i kitea i roto i nga pūtau granulosa ovarian he poto ake i tera i kitea i roto i nga chondrocytes, e whakaatu ana i te mana o te mahi biosynthetic proteoglycan i roto i te katoa o nga mahi metabolic o nga pūtau.
