Hua

APS-5 Ko te tïpako chemiluminescent i runga i 9,10-dihydroacridine, e te nuinga whakamahia mo ELISA rapunga o pūhui ki alkaline phosphatase AP conjugates;9,10-dihydroacridine i roto i te hanganga o te APS-5 Ka taea e te hanganga hydroacridine te tuku marama tonu, te pumau me te pai i raro i te mahi a AP, me te APS-5 e tino pai ana mo te whakamatautau otinga ngohe phosphatase me te immunoassay hono-honohono phosphatase.

Ka whakamahia hei rangahau mo nga maramara ira.Ka tapaina te tauhohenga ki te acridan (9,10-dihydroacridine) hei tïpako me te phosphatase kawakore, e whakaputa ana i te chemiluminescence kaha teitei.Ka whakarato i te tairongo pai ake me te ngawari o te whakamahi mo te whakakotahitanga phosphatase alkaline i te wa e kitea ana te chemiluminescent.Ka kitea te phosphatase alkaline i roto i te iti iho i te 10-19 mol, ka tere te tihi ki te whakaiti i te wa kitenga me te whakanui ake i te whakaputanga, a, ko te pikinga o te arai whakatikatika raina ka tuhia ki te taurangi rite ki te 1.0.Kotahi te rahinga, nui ake ranei o te enzyme e whakaputa ana i te rahinga kotahi, neke atu ranei o te whakamarama, te whakamarama tonu—kaore i te tino tono mo te wa whakamatautau.Ka taea te panui i te kaha o te rama mai i te kurupae whakatika raina i nga wa katoa, a, ko nga hua tātari karekau e aro ki te pāmahana i roto i te awhe o te 22°C - 35°C, ka whakaiti i te tika e hiahiatia ana hei whakahaere i te pāmahana.

painga:

1 Te tairongo teitei - ka kitea nga tohu pūmua i raro i te 5pg;

2 Te kaha o te rama - ka taea te eke ki te tihi o te rama i roto i te wa poto;

3 Te marama tonu me te pumau - kaore e pa ki te pāmahana i roto i te 25-35 ℃, karekau e hiahia ki te whakahaere i te pāmahana.

Whakatauritenga tono o nga taputapu whakamarama APS-5 (193884-53-6) me AMPPD (122341-56-4)

Ko te APS-5 (193884-53-6) me te AMPPD (122341-56-4) he taputapu chemiluminescent mo te alkaline phosphatase, a ko enei e rua e kiia ana ko nga reagents detection sensitivity ultra-high, no reira ko enei reagents e rua kei te kitea Ko tehea te mea pai ake ko tehea te mea kino?Me pehea te whiriwhiri i te wa e whakamahi ana?

I tetahi taha, i te wa e whakamahia ana te APS-5 hei taputapu tirotiro phosphatase alkaline, ka taea e te arokitenga te tae ki te 10-19 te kukū molar, ka taea e te waa whanaketanga tae te eke ki te tihi teitei i roto i te 10 hēkona, ka roa te roa o te rewharewha. wā.I roto i enei ahuatanga, ka taea te kii he tino pai nga taonga a APS 5.Heoi, he iti noa te APS-5 i roto i te pāmahana tono, me te pai ake o te pāmahana whakaatu tae i raro i te 20'C.I tetahi atu ringa, ka whakamahia te AMPPD hei taputapu rapu mo te alkaline phosphatase, ka kiia hoki ko te maaramatanga o te kitenga he tino teitei, engari kaore e taea te eke ki te taumata o te APS 5. Ko te wa whanaketanga tae ka tae ki te tihi teitei i te 30 hēkona. , a ka roa ake te roanga o te rangi.He tino pai te AMPPD ki nga ahuatanga o runga ake nei, engari he iti ake i te APS5, engari ko te pāmahana whakakitenga tae kaore i te tino rite ki te APS5, he maamaa ake te pāmahana rokiroki o 2--8C i te APS-5.Hei whakarāpopototanga, ahakoa he nui ake te APS-5 i te AMPPD i te tika o te rapunga, ka ngaro te pumau.Me pehea te whiriwhiri ka whakawhirinaki ki nga hiahia motuhake o te kitenga.

1. Whakataki o APS-5 (193884-53-6):

Ingoa Ingarihi: APS-5

Nama CAS: 193884-53-6

tātai rāpoi: C21H15ClNO4PS Na2

Te taumaha ngota: 489.819

Te ahua: te paura totoka ma-kore

Ihirangi: ≥98%

Whakatakotoranga: 1g, 5g, 10g

Nga tikanga rokiroki: -20 ℃ tiaki

(4-Chlorophenylmercapto)(10-methyl-9,10-dihydroacridine methylene) te tote disodium phosphate, e kiia ana ko APS-5, he reagent chemiluminescent ka whakamahia hei taputapu chemiluminescent.Ka whakarato i te tairongo pai ake me te ngawari o te whakamahi mo te whakakotahitanga phosphatase alkaline i te wa e kitea ana te chemiluminescent.Ka tapaina te tauhohenga ki te acridan (9,10-dihydroacridine) hei tïpako me te phosphatase kawakore, e whakaputa ana i te chemiluminescence kaha teitei.Ko te APS-5 he reagent pai mo te whakatau i te mahi phosphatase na roto i nga whakamatautau otinga me nga immunoassays e hono ana i te enzyme phosphatase.Ka rite ki te tïpako rama, APS-5 he tairongo rapu tiketike me te wa tauhohenga poto.

2. Nga korero whakataki mo te AMPPD (122341-56-4)

Ingoa Ingarihi: AMPPD

Nama CAS: 122341-56-4

tātai rāpoi: C18H23O7P

Te taumaha ngota: 382.344781

Te ahua: te paura ma

Maama: ≥97%

Whakatakotoranga: 1g, 5g, 10g

Nga tikanga rokiroki: -20 ℃ tiaki

Whakatupatotanga: Me maroke kia kore e rewa me te lyophilization