Hua

Ka noho tonu te Mycobacterium tuberculosis mo nga tekau tau i roto i te kaihautu tangata.Ko nga huarahi whakautu kaha e whai waahi ana ki te polyphosphate parakore [poly(P)], ka whakahiatohia, ka whakahiatohia e te polyphosphate kinase (PPK) me te exopolyphosphatase (PPX), e whakaponohia ana ka whai waahi nui ki te whakahaere i te mau tonu o te huakita.E whakaatu ana matou i konei ko te whakaemi M. tuberculosis poly(P) he hononga-a-te-taapiri ki te aukati i te tipu o te bacillary.Ka tautuhia ano e matou a M. tuberculosis Rv1026 hei exopolyphosphatase hou me te mahi hydrolytic ki te poly(P) mekameka roa.Ma te whakamahi i te punaha korero tetracycline-inducible ki te turaki i te whakahuatanga o Rv1026 (ppx2), i kitea e matou ko te whakaemi M. tuberculosis poly(P) ka arahi ki te puhoi o te tipu me te whakaheke i te whakaraeraetanga ki te isoniazid, te kaha ake o te atete ki te wera me te waikawa pH, me te pai ake o te oranga o roto i roto i nga pūtau. i roto i te mate macrophage.Na roto i te tuku irahiko moroiti, te ppx2 knockdown riaka i whakaatu te nui haere o te matotoru pakitara o te pakitara, e hono ana ki te whakahekenga o te pakitara o te pakitara o te pakitara ki nga raau taero hydrophilic, kaua ki te whakauru i nga papu efflux tarukino me te whakarereketanga o te hanga biofilm e pa ana ki te mana vector kau.I kitea e te tātaritanga whakawhiti me te metabolomic te hekenga tinana o te paheketanga ppx2 e tohuhia ana e te whakahekenga o te tuhi me te whakamaori me te hekenga o nga taumata glycerol-3-phosphate.Hei whakarāpopototanga, ka whai wāhi nui te poly(P) ki te aukati i te tipu o te mate kohi M. me te heke o te metabolic me te whai waahi ki te manawanui paturopi na roto i te whakarereketanga o te uruhanga pakitara o te pakitara. )ppGpp, e whakaponohia ana hei takawaenga i te mau tonu o te Mycobacterium tuberculosis.I roto i tenei rangahau, i tautuhia e matou he whaariki hou (Rv1026, PPX2) te kawenga mo te whakamaarama i te poly (P) mekameka roa.Ko te riaka M. tuberculosis i hangaia he ngoikore i roto i te ira ppx2 i whakaatu i te pikinga o te poly(P), i whai hononga ki te hopukanga o te tipu o te huakita moata me te whakaiti i te pai ki te rongoa isoniazid raina tuatahi, me te piki ake o te oranga huakita i te wa e pa ana ki te ahotea. nga ahuatanga me roto i nga macrophages.E pa ana ki te riaka mana whakahaere, i whakaatu te mutant i te kaha ake o te matotoru o te pakitara o te pūtau me te whakaheke i te uruhanga tarukino.Ko te whakaputanga ira o te ao me te tātari metabolite i kitea te iti o te whakaputanga o te miihini whakawhiti me te miihini whakawhiti me te huringa o te whakamahi puna waro.Hei whakarāpopototanga, he mea nui te whakaritenga o te toenga poly(P) mo te tohe tonu o te mate kohi M.