rongo

He maha nga toa kare i waiatatia huri noa i a tatou, he ahua noa nei, engari ko te tikanga he nui ta ratou takoha ki a tatou.Ko te Proteinase K te "toa karekau" i roto i te umanga tātaritanga rapoi, ahakoa ka whakatauritea ki te "nui me te kaha" i roto i te umanga, he iti te matua o te proteinase K kua roa kua warewarehia e tatou tona hiranga.Na te pakarutanga o te mate hou o te karauna, kua piki te tono mo te proteinase K, a, ko te toha ki te kainga me waho kei muri rawa atu i te kai, a ka mohio nga tangata katoa he mea nui te proteinase K.
He aha te whakamahinga o te proteinase K
Ko te Proteinase K he protease serine me te mahi enzyme proteolytic ka taea te pupuri i nga mahi i roto i te tini o nga taiao (pH (4-12.5), te tote teitei, te teitei o te wera o te 70 ° C, me etahi atu).I tua atu, ko te mahi o te proteinase K kaore i te whakakorehia e te SDS, te urea, te EDTA, te guanidine hydrochloride, te guanidine isothiocyanate, me etahi atu, me te nui o te horoi ka taea hoki te whakarei ake i te mahi o te proteinase K. I roto i te maimoatanga hauora (te huaketo me te whakakorenga microbial. ), te kai (meat tenderization), te hiako (te whakamarumaru o nga makawe), te hanga waina (te whakamarama waipiro), te whakarite waikawa amino (nga huruhuru whakaheke), te tangohanga waikawa nucleic, in situ hybridization, etc., proteinase K He tono.Ko te tono tino whakamahia ko te tangohanga waikawa nucleic.
Ka taea e te Proteinase K te whakaeneene i nga momo pūmua katoa i roto i te tauira, tae atu ki era histones e herea ana ki nga waikawa karihi, kia puta ai nga waikawa karihi mai i te tauira ka tukuna ki roto i te tangohanga, hei whakahaere i te mahi tangohanga me te purenga.I te kitenga o te waikawa nucleic viral, ko te proteinase K tetahi o nga waahanga nui i roto i te otinga whakatauira huaketo.Ka taea e te Proteinase K te pakaru me te whakakore i te pūmua koti o te huaketo, he pai ake i te wa o te kawe me te kimi;i tua atu, ka taea hoki e te proteinase K te whakaheke i te RNase te aukati i te paheketanga o te RNA viral me te awhina i te kitenga waikawa karihi.
Ko te rongonui mo te po o te proteinase K
Ahakoa i roto i te mara rangahau pūtaiao, i roto ranei i te mara o te IVD, ko te tangohanga waikawa karihi te tino whakamatautau, no reira ko te proteinase K he mea tino nui tonu.Heoi, i nga wa o mua, ko te proteinase K he iti rawa te mohio ki tana mahi.Ko tetahi waahanga nui o tenei na te mea he tino pumau te hononga tuku me te tono o te proteinase K.He ruarua nga tangata ka whakaaro he raru te tuku o te proteinase K.
Na te pakarutanga o te mate hou o te karauna, kua piki te hiahia mo te whakamatautau waikawa karihi.I te mutunga o Hune 2020, kua oti i Haina te tata ki te 90 miriona nga whakamatautau karauna hou, a he tino whakamataku tenei nama i te ao.I roto i nga whakamatautau tangohanga waikawa karihi, he 50-200 μg/mL te kukū mahi o te proteinase K.Ko te tikanga, tata ki te 100 μg o te proteinase K hei tango i tetahi tauira o te waikawa karihi.I roto i te whakamahi tūturu, ki te whakapiki ake i te pai o te tangohanga waikawa nucleic, he maha nga wa ka whakamahia te Proteinase K i roto i te nui ake o te nui.Ko te kitenga waikawa nucleic o te coronavirus hou kua nui te tono proteinase K.Ko te toenga o te tuku me te tono tuatahi o te pūmuaase K i whati wawe, ka noho ko te pūmuaase K hei taonga aukati mate uruta i te po.
He uaua ki te hanga proteinase K
Ahakoa i te whanaketanga o te mate urutaru, ko te uara nui o te proteinase K kua whakanuia e te tangata, he mea whakama na te nui o te iti-matua o te proteinase K, he iti noa nga kamupene o te kaainga kua uru ki te hanga proteinase K. e hiahia ana ki te whakatu i te hanga pūmua K i roto i te tukanga whakaputa, i kitea ko te proteinase K he pūmua tino motuhake.He tino uaua ki te whakawhanui i te kaha whakangao o te proteinase K i roto i te wa poto.
Ko te hanga nui o te proteinase K ka pa ki nga uaua e whai ake nei
1. He korero iti
Ka taea e te Proteinase K te whakaheke i te nuinga o nga pūmua me te whakararu kino ki te pūtau manaaki korero.No reira, he iti rawa te taumata korero o te proteinase K.Ko te tirotiro i nga punaha korero me nga riipene e tino whakaatu ana i te proteinase K me roa ake te huringa.
2. Ko te toenga o te poaka me te waikawa nucleic
Ko te FERMENTATION nui-nui ka whakauru i te nui o te poaka me nga toenga waikawa karihi manaaki.He uaua ki te tango i enei poke me te mahinga ngawari o te purenga, me te purenga uaua ka piki ake te utu me te whakaheke i te tere whakaora.
3. Te korekore
Ko te Proteinase K kaore i te tino pumau, ka taea e ia te enzymolyze i a ia ano, a he uaua ki te penapena i runga i te 37°C mo te wa roa kaore he kaihoko tiaki.
4. He ngawari ki te whakaheke
I te wa e whakareri ana i te paura whakamaroke-maroke o te proteinase K, kia mohio ai he nui te ihirangi totoka o te proteinase K i roto i te paura whakamaroke-tio, he mea tika ki te taapiri i tetahi kaihoko whakamarumaru-maroke i te kukū teitei, engari ka Ko te kukū o te pūmuaase K ka tae ki te 20mg/mL me runga ake, he ngawari Ko te whakahiatotanga ka hanga he para, he tino uaua ki te whakamaroke-tio o te pūmuaase K me te kiko totoka teitei.
5. Te haumi nui
Ko te Proteinase K he kaha te mahi poroteasee, a ka taea e ia te whakamaarama i etahi atu parea i roto i te taiwhanga.No reira, ka hiahia te proteinase K ki nga waahi whakaputa motuhake, taputapu, me nga kaimahi mo te rangahau me te whakawhanaketanga me te whakaputa.
XD BIOCHEM's proteinase K otinga
XD BIOCHEM has a mature protein expression and purification platform, and has rich experience in the expression and purification of recombinant proteins and optimization of production processes. Through the rapid formation of a research and development team, the large-scale production process of proteinase K has been overcome. The monthly output of freeze-dried powder is more than 30 KG. The product has stable performance, high enzyme specific activity, and no host cytochrome and nucleic acid residues. Welcome to contact XD BIOCHEM Obtain a trial package (E-mail: sales@xdbiochem.com Tel: +86 513 81163739).
Ko nga otinga hangarau a XD BIOCHEM kei roto
Ma te whakamahi i te whakauru plasmid maha-kape, ka tohua nga riipene korero teitei me te taumata whakapuaki o te 8g/L, e hinga ana i te raruraru o te taumata whakapuaki iti o te proteinase K;
Na roto i te whakaturanga o te tukanga purenga maha-taahiraa, i pai te tango i te cytochrome ope me nga toenga waikawa karihi o te proteinase K i raro i te uara paerewa;
Na roto i te tirotiro teitei o nga hanganga parepare whakamarumaru, ka kowhiria he parepare ka taea te pupuri i te proteinase K i te 37°C;
Ka taea e nga kaiwhaiwhai tirotiro te raru he ngawari te whakahiato me te whakaheke i te poroteina K i nga kukū teitei, me te whakatakoto i te turanga mo te whakamaroke whakatio i nga ihirangi totoka teitei o te proteinase K.

XD BIOCHEM proteinase K tauira

XD BIOCHEM proteinase K whakamātautau pūmautanga: karekau he huringa nui o te mahi i muri i te 80 d i te pāmahana rūma

XD BIOCHEM proteinase K whakamātautau pūmautanga: karekau he huringa nui o te mahi i muri i te 80 d i te pāmahana rūma

Te whakatairitetanga o te paanga tangohanga waikawa karihi o XD BIOCHEM proteinase K me nga hua whakataetae.I roto i te tukanga tangohanga waikawa karihi, ka whakamahia te XD BIOCHEM me te proteinase K whakataetae.Ko te kaha o te tangohanga o te XD BIOCHEM proteinase K he teitei ake, he iti ake te uara Ct o te ira e whai ana.