ONPG CAS:369-07-3 98.0% Min White Ki te Wehe -Paura Ma
| Nama Putumōhio | XD90006 |
| CAS | 369-07-3 |
| Ingoa Hua | ONPG(2-Nitrophenyl-beta-D-galactopyranoside) |
| Tātai rāpoi | C12H15NO8 |
| Taumaha rāpoi | 301.25 |
| Nga Taipitopito Rokiroki | 2 ki te 8 °C |
| Waehere Rere Whakariterite | 29400000 |
Whakatakotoranga Hua
| Pure (HPLC) | Min.98.0% |
| Te ahua | Ma ki te paura - ma te paura |
| Rongoā(1% i te Wai) | He maamaa, he kore tae ki te otinga paku kowhai |
| Ihirangi wai(Karl Fisher) | Max.0.5% |
| Tītaka whatu tauwhāiti [α]D20(c=1, H2O) | - 65.0 ° C ki -73.0 ° C |
Matapaki mo te whakamatautau ONPG (whakamatautau β-galactosidase)
He maha nga patai i puta ina tata nei: 1. He aha te take ka whakamahia te whakamatautau ONPG hei tohu i te whakaroa o te lactose FERMENTATION?2. He aha te take i kii ai te paerewa o te motu e tika ana kia whakamahia te 3% konutai konutai trisaccharide rino (te rino trisaccharide ranei) mo te whakamatautau ONPG?3. Mo te Vibrio parahaemolyticus, i te wa e whakahaere ana i te whakamatautau OPNG, he aha te take me tapiri atu ai te toluene ki te maturuturu kia rite ki te paerewa?He aha te mahi?
He maha nga korero kua arotakehia e ta maatau kamupene, kua whakarapopotohia, kua tohatohahia ki a koe i raro nei:
Kaupapa: Ko te ingoa Hainamana o ONPG he o-nitrobenzene-β-D-galactopyranoside.Ka taea e te β-galactosidase te whakamaroke i te ONPG ki te galactose me te o-nitrophenol (ONP) kowhai, no reira ka kitea te mahi o te β-galactosidase e te huringa tae o te reo ahurea.
Ko te Lactose he huka e tika ana kia kitea e te nuinga o nga microorganisms.E rua nga whākōkī e hiahiatia ana mō tōna pākia, ko tētahi he pūtau pūtau, ka uru te lactose ki ngā pūtau i raro i te mahi a te permease;ko tetahi atu ko te β-galactosidase, e whakamaua ana te lactose ki te galactose.Lactose me te hukahuka.Ka taea hoki e te β-Galactosidase te mahi tika i runga i te ONPG ki te hauwai ki te galactose me te kowhai o-nitrophenol (ONP).Ka taea te mahi i roto i te 24 haora, ahakoa me te lactose whakaroa fermenters.Na reira, ka whakamaramatia nga hua kitenga o te kohi i te ahurea 1 mai i te agara me te wero i roto i te porowhita katoa i te 36°C mo te 1-3h me te 24h.Mena ka puta te β-galactosidase, ka huri kowhai i roto i te 1-3h, mena karekau he enzyme penei, kaore e rereke te tae i roto i te 24h.
E ai ki nga enzymes e rua i runga ake nei, ka taea te wehewehe nga microorganisms ki nga waahanga e whai ake nei:
1 lactose-fermenting (18-24 haora) huakita me te permease me te β-galactosidase P + G +;
2 Te whakaroa i te lactose fermenters (he roa ake i te 24 haora) karekau he urunga engari he galactosidase: P-G+.
3 Ko nga mea whakapoipoi kore-lactose e kore e rua te permease me te galactosidase: P- G-.
Ka taea te whakamahi i te whakamatautau ONPG ki te wehewehe i te huakita lactose-lag-fermenting (PG+) mai i te huakita lactose kore-fermenting (PG-), penei:
1 Whakawehewehe i nga kaiwhangai lactose mutunga (P- G+) mai i nga fermenter kore-lactose (P- G-).
(a) Citrobacter (+) me Salmonella arizonae (+) mai i Salmonella (-).
(b) Escherichia coli (+) no Shigella sonnei (-).
He aha te take i mahia ai te whakamatautau ONPG ma te whakamahi i te ahurea mo te po i runga i te 3% konutai pūhaumāota ferric trisaccharide (rino trisaccharide)?He maha nga korero kua korerohia e ta maatau kamupene, engari kaore he korero marama.I runga i te paetukutuku FDA anake, kua tuhia e "Inoculate cultures to be tested on triple sugar iron agar slants and incubated for 18 h i 37°C (tetahi atu pāmahana e tika ana, ki te hiahiatia) . Nutrient (etahi atu) agar slants kei roto 1.0 Ka taea hoki te whakamahi % lactose."Ko te tikanga: ka werohia nga huakita whakamatautau ki te rino trisaccharide me te whakatipu i te 37°C mo te 18h.Ka whakaaetia hoki te 1% o te lactose ki roto i nga maara agar (tetahi atu ranei).No reira, ka whakatauhia he lactose kei roto i te reo rino trisaccharide.Whai muri i te tipu o te po, kua hua mai e nga huakita he β-galactosidase kaha.Ma te whakamahi i aua huakita, ka tere ake te pirau o ONPG e te β-galactosidase.He tere ake, he pai ake te whakaatu o te ahuatanga whakamatautau.I tua atu, ko te taapiri o te toluene me te pati wai mo te 5 meneti he mea katoa kia puta katoa te β-galactosidase.
