Hua

He microRNA tino tairongo (miRNA) biosensor e whakamahi ruthenium oxide nanoparticle (RuO(2) NP)-i timatahia te polymerization o 3,3'-dimethoxybenzidine (DB) me te miRNA-whakaahua te waipara o te poly insulating (3,3'-dimethoxybenzidine) (PDB) kiriata e whakaahuatia ana i roto i tenei mahi.I hangaia te kaikoiora mai i te monolayer whakauru o te oligonucleotide hopu probes (CPs) me te 4-mercaptoaniline i runga i te hiko koura.Whai muri i te whakakotahitanga me te RuO(2) NP-tagged target miRNA, he ranunga o DB/H(2)O(2) i roto i te pH 5.0 0.10 M acetate buffer i tukuna ki te koiora.Ko nga RuO(2) NP ka mahi hei kaikawe / whakakorikori polymerization mo te polymerization o DB.A ko nga whenu miRNA ranu me nga CP koreutu hei tauira, hei arahi i te tukunga o te PDB.Ko te nui o te PDB kua penapena me tona mana whakaahuru e hono tika ana ki te kukū o te miRNA whainga i roto i te otinga.I kitea e nga whakamatautau hiroki hiko impedance impedance he hononga ahorangi-whakawhitinga parenga-whakaaro mai i te 6 .0 fM ki te 2.0 pM i tutuki i muri i te 60 meneti o te heki i roto i te ranunga DB/H(2)O(2).Karekau he whakawhitinga-whakawhitiwhiti i waenga i mua i te miRNA me te miRNA pakeke, me te iti rawa o te whakawhiti whakawhiti i waenga i nga mema o te whanau miRNA e tata ana, ahakoa i nga taumata kotahi-kore-kore.Ko tenei kaikoiora-a-impedance e tuku ana i tetahi waahanga ataahua mo te whakaputa korero miRNA ka taea te whakawhanake i te punaha whakaata miRNA maha kawe.