Streptomycin sulfate CAS:3810-74-0 Te paura ma
| Nama Putumōhio | XD90361 |
| Ingoa Hua | Streptomycin sulfate |
| CAS | 3810-74-0 |
| Tātai rāpoi | (C21H39N7O12)2·3H2SO4 |
| Taumaha rāpoi | 1457.39 |
| Nga Taipitopito Rokiroki | 2 ki te 8 °C |
| Waehere Rere Whakariterite | 29412080 |
Whakatakotoranga Hua
| Kōeke | USP36/EP8.0 |
| pH | 4.5-7.0 |
| Ngaronga i te whakamaroke | ≤7.0% |
| Te whakamatautau | ≥720IU/MG |
| pūkawa | 18.0~ 21.5% |
| Te ahua | Te paura ma, tata ma ranei, tino memeha ki te wai, tata kore e rewa ki te waihano. |
| Mewaro | ≥0.3% |
| Streptomycin B | ≤3.0% |
| Whakamātautau Tae | ≥90.0% |
| Waikawa whanariki | ≤1.0% |
| Ko te konutai konutai konutai pūkawa | ≤0.4% |
Ahakoa kaore i te tino mohiohia te takenga o te whakahekenga o te kōpae intervertebral, ko tetahi huarahi hei aukati i tenei mahi ko te aukati i te apoptosis.I roto i te rangahau o naianei, i tirotirohia nga paanga anti-apoptotic o te carboxymethylated chitosan (CMCS) i roto i nga pūtau nucleus pulposus (NP) me te whai ki te whakarei ake i te oranga o nga pūtau kōpae.Ko nga pūtau NP kiore ka wehea, ka whakatipuhia i roto i te vitro, ka whakamahia te hauwai (H2O2) hei hanga i te tauira apoptosis pūtau NP.I aromatawaihia te oranga o te pūtau ma te kete tatau tatau-8.Ko te ōwehenga o nga pūtau apoptotic i rangahauhia e te annexin V‑fluorescein isothiocyanate (FITC) me te propidium iodide (PI) tātaritanga whakakikorua rua, a ko te ahua o te ahua i kitea e te Hoechst 33342 whakakikorua.Ko te kaha o te membrane mitochondrial o nga pūtau NP i aromatawaihia e te rhodamine 123 fluorescence staining.Ko te whakamaoritanga whakamuri (RT)-tauhohenga mekameka polymerase (qPCR) i mahia hei ine i nga taumata mRNA o te synthase nitric oxide inducible (iNOS), te caspase-3, te lymphoma pūtau-B (Bcl)-2, te momo II collagen me te aggrecan.I mahia te wetewete o te Western blot kia kitea nga taumata pūmua o te iNOS me te Bcl‑2.Ko te apitihanga V‑FITC/PI me te Hoechst 33342 hua whakakikorua i tohu i taea e CMCS te aukati i nga pūtau NP mai i te apoptosis i runga i te tikanga whakawhirinaki.Rhodamine 123 staining i whakamarama i whakaitihia e te CMCS te ngoikoretanga o te kaha o te membrane mitochondrial i roto i nga pūtau NP e tukinotia ana e H2O2.Ko te whakahekenga o te caspase‑3 me te nui ake o te mahi Bcl‑2 i kitea i roto i nga ruma NP-whakaritea a CMCS e te RT‑qPCR me te wetewete o te uru.Ko te CMCS ano i whakatairanga i te tipu me te hunanga o te momo II collagen me te aggrecan i roto i te H2O2-treated NP cells.I tohuhia te CMCS ki te whai hua ki te aukati i te mate o nga pūtau apoptotic in vitro, e whakaatu ana i nga painga pea o tenei huarahi rongoa ki te whakahaere i te whakahekenga o te kōpae.
