Hua

Reagent wairewa-wai mo te whakatau photometric enzymatic o te hauwai hauwai.Ko nga reagents hou a Trinder he tino pärönaki aniline wairewa-wai e whakamahia nuitia ana i roto i nga whakamatautau tātaritanga me nga whakamatautau koiora.He maha nga painga i runga i nga reagents chromogenic tikanga i roto i te whakatau tae o te mahi hauwai peroxide.Ko nga reagents hou a Trinder he tino pumau hei whakamahi i roto i nga otinga me nga punaha rapunga paipa whakamatautau.I te aroaro o te hydrogen peroxide me te peroxidase, i whakaatuhia te reagent a Trinder ki te 4-aminoantipyrine (4-AA) me te 3-methylbenzothiazole sulfonehydrazone (MBTH) i te wa o te tauhohenga hononga oxidative.Ka hangaia he waikano puru, puru ranei.Ko te molar absorbance o te waikano i honoa ki te MBTH he 1.5-2 teitei ake i tera o te tae i honoa ki te 4-AA;engari, ko te otinga 4-AA he pai ake i te otinga MBTH.Ko te tïpako he waikura enzymatically e tona oxidase ki te whakaputa hauwai hauwai.He rite te kukū hauwai ki te kukū tïpako.Na reira, ka taea te whakatau i te nui o te tïpako ma te whanaketanga o te tae o te tauhohenga hononga oxidative.Ka taea te whakamahi i te huka, te waipiro, te acyl-CoA me te cholesterol ki te kite i aua taputapu kua honoa ki te reagent a Trinder me te 4-AA.E 10 nga reagents hou a Trinder e waatea ana.I roto i nga reagents hou a Trinder, ko TOOS te mea tino whakamahia.Heoi, mo tetahi tïpako motuhake, he mea tika kia whakamatauria nga karaehe rereke o nga reagents pukapuka a Trinder hei whakawhanake i te punaha rapunga tino pai.

Reagent wairewa-wai mo te whakatau photometric enzymatic o te hauwai hauwai.Ko nga reagents hou a Trinder he tino pärönaki aniline wairewa-wai e whakamahia nuitia ana i roto i nga whakamatautau tātaritanga me nga whakamatautau koiora.He maha nga painga i runga i nga reagents chromogenic tikanga i roto i te whakatau tae o te mahi hauwai peroxide.Ko nga reagents hou a Trinder he tino pumau hei whakamahi i roto i nga otinga me nga punaha rapunga paipa whakamatautau.I te aroaro o te hydrogen peroxide me te peroxidase, i whakaatuhia te reagent a Trinder ki te 4-aminoantipyrine (4-AA) me te 3-methylbenzothiazole sulfonehydrazone (MBTH) i te wa o te tauhohenga hononga oxidative.Ka hangaia he waikano puru, puru ranei.Ko te molar absorbance o te waikano i honoa ki te MBTH he 1.5-2 teitei ake i tera o te tae i honoa ki te 4-AA;engari, ko te otinga 4-AA he pai ake i te otinga MBTH.Ko te tïpako he waikura enzymatically e tona oxidase ki te whakaputa hauwai hauwai.He rite te kukū hauwai ki te kukū tïpako.Na reira, ka taea te whakatau i te nui o te tïpako ma te whanaketanga o te tae o te tauhohenga hononga oxidative.Ka taea te whakamahi i te huka, te waipiro, te acyl-CoA me te cholesterol ki te kite i aua taputapu kua honoa ki te reagent a Trinder me te 4-AA.E 10 nga reagents hou a Trinder e waatea ana.I roto i nga reagents hou a Trinder, ko TOOS te mea tino whakamahia.Heoi, mo tetahi tïpako motuhake, he mea tika kia whakamatauria nga karaehe rereke o nga reagents pukapuka a Trinder hei whakawhanake i te punaha rapunga tino pai.