X-GAL CAS:7240-90-6 98% Ma ki te paura tioataata-ma
| Nama Putumōhio | XD90008 |
| Ingoa Hua | X-Gal (5-Bromo-4-chloro-3-indolyl-beta-D-galactopyranoside) |
| CAS | 7240-90-6 |
| Tātai rāpoi | C14H15BrClNO6 |
| Taumaha rāpoi | 408.63 |
| Nga Taipitopito Rokiroki | -2 ki te -6 °C |
| Waehere Rere Whakariterite | 29400000 |
Whakatakotoranga Hua
| Te ahua o te otinga | He maamaa, kore tae ki te otinga kowhai marama (50mg/ml i DMF:MeOH, 1:1) |
| Te hurihanga whatu motuhake | -61.5 +/- 1 |
| Te ahua | Ma ki te paura tioata ma-kore |
| HPLC pure | min 99% |
| Wairewa (5 % i DMF) | Wairewa (5% w/v,DMF) |
| Wai KF | mōrahi 1% |
| Whakamātautau (HPLC i runga i te Waiwai) | min 98% w/w |
Whakamahinga o X-gal
Ko te X-gal (he whakapoto BCIG mo te 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside) he puhui waro kei roto i te galactose e hono ana ki te indole whakakapi.I whakahiatotia e Jerome Horwitz me ona hoa mahi te puhui i te tau 1964. Ko te ingoa matū okawa ka whakapotohia ki te iti o te tika engari he iti ake te uaua o nga kianga penei i te bromochloroindoxyl galactoside.Ko te X mai i te indoxyl pea te puna o te X i roto i te whakawhitinga X-gal.He maha nga wa ka whakamahia te X-gal i roto i te koiora irapoi hei whakamatautau mo te noho mai o tetahi enzyme, β-galactosidase, hei whakakapi i tana whaainga o mua, he β-galactoside.Ka whakamahia ano hoki hei kite i te mahi o tenei whaakiki i roto i te matū matū me te huakita.Ko te X-gal tetahi o te maha o nga glycosides indoxyl me nga hākawa e tuku ana i nga pūhui kahurangi korekore e rite ana ki te waikano indigo i te hua o te hauwai-catalyzed hydrolysis.
Ko te X-gal he tairitenga o te lactose, na reira ka whakamaaramahia e te enzyme β-galactosidase e wawahi ana i te hononga β-glycosidic i roto i te D-lactose.Ko te X-gal, ka wehea e te β-galactosidase, ka puta he galactose me te 5-bromo- 4-chloro-3-hydroxyindole - 1. Ko te mea whakamutunga ka rearua noa, ka waikura ki te 5,5'-dibromo-4,4'-dichloro -indigo - 2, he hua tino kikorangi e kore e rewa.Ko te X-gal ake he kore tae, no reira ka whakamahia te ahua o te hua puru-tae hei whakamatautau mo te noho kaha o te β-galactosidase.Ma tenei ka taea te β-galactosidase huakita (e kiia nei ko lacZ ) hei kairipoata mo nga momo tono.
I roto i te tātaritanga kakano-rua, ka whakamahia pea te β-galactosidase hei kairipoata ki te tautuhi i nga pūmua e pahekoheko ana tetahi ki tetahi.Ma tenei tikanga, ka tirotirohia nga whare pukapuka ira mo te taunekeneke pūmua ma te rewena, te punaha huakita ranei.Ina he pai te taunekeneke i waenga i nga pūmua e tirotirohia ana, ka puta he herenga o te rohe whakahohe ki tetahi kaiwhakatairanga.Mena ka honohia te kaiwhakatairanga ki te ira lacZ, ko te hanga o te β-galactosidase, ka puta mai i te hanganga o nga koroni puru-pigmented i te aroaro o X-gal, na reira ka tohu he pai te taunekeneke i waenga i nga pūmua.Ka whakawhäitihia pea tënei tikanga ki te tirotiro i nga whare pukapuka he iti iho i te 106. Ko te wehenga angitu o te X-gal ka puta he haunga kino na te pähekeheke o te indole.
I te mea ko te X-gal ake he kore tae, ka whakamahia pea te waahi o te hua puru-tae hei whakamatautau mo te noho o te β-galactosidase kaha.
Ko tenei tohu ngawari o te enzyme kaha ka taea te whakamahi i te ira mo te βgalactosidase (te ira lacZ) hei ira kairipoata i roto i nga momo tono.
