Hua

CTP: Ko te phosphocholine cytidylyltransferase α (CCTα) he whākōkī karihi e whakakorikori ana i te takahanga reiti i roto i te ara CDP-choline mo te whakahiato phosphatidylcholine (PC).Ko te whakahoahoatanga o te lipid o CCTα ka puta tana whakawhiti ki te kopaki karihi me te roha o te whatunga membrane intranuclear e kiia nei ko te nucleoplasmic reticulum (NR) na tetahi tikanga e pa ana ki te huringa membrane.Kei te hiahiatia ano nga lamins karihi mo te pumau me te whakanui i te NR, engari ko tenei hanganga ahurei, ko te lamina karihi ranei i te nuinga o te waa, e hiahiatia ana mo te whakahiato PC kaore i te mohiotia.Hei tirotiro i tenei hononga, i whakahekehia te lamina karihi e te RNAi, i whakakorehia ranei e te whakaaturanga o te Hutchinson-Gilford progeria syndrome (HGPS) mutant lamin A (progerin), a ka tātarihia te paanga ki te CCTα me te choline metabolism.Ko te whakamutua o te lamin A/C, te lamin B1 ranei i roto i nga pūtau CHO ki te whakaiti i te NR kaore he painga ki te whakahiato PC, ko te patoto-rua kaore i aukati i te huarahi.Ma te whakapumau i tana mahi iti i roto i te whakahiato PC, ko te 10% anake o te choline / ethanolamine phosphotransferase i kitea i roto i te NR.I roto i nga pūtau CHO, ko te CCTα he nucleoplasmic me te noho tahi me te GFP-progerin i roto i nga kopa karihi me nga whakaurunga;heoi, i whakaatuhia e nga fibroblasts HGPS he tohatoha rereke o CCTα i roto i te cytoplasm me te kopaki karihi i haere tahi me te whakahekenga 2-whakaiti i te whakahiato PC.Ahakoa te whakarereketanga o te waahi, i kitea e nga whakamatautau tohu tohu koina kaore i pa te mahi CCT, a ko te aukati i te whakahiato PC i tohuhia ki te whakahekenga o te mahi a te kaikawe choline hemicholinium-tairongo.Ka whakatauhia e maatau te mahi tahi a CCTα me nga lamins ki te hanga i te NR, engari ko te hanganga whanui o te envelopu karihi he iti te paanga ki nga mahi CCT me te whakahiato PC.