Hua

He rite ki etahi atu wheori RNA pai-waa, ka tukuna nga tombusvirus e te maataki-a-membrane (VRC).Kei roto i te VRC ko te RNA polymerase (RdRp), te RNA polymerase (RdRp), te p33 RNA chaperone, me te maha o nga pūmua kaihautu i whiriwhiria.Kia noho hei RdRp mahi i muri i tana whakamaoritanga, me whakauru te p92 replication protein ki roto i te VRC, ka whai muri i tana whakahohenga.Kua whakaatuhia e matou i mua i roto i tetahi whakamatautau i runga i te tango rewena kore-puu, ko te whakahoetanga o te Tomato bushy stunt virus (TBSV) RdRp e hiahia ana ki tetahi tauwehe kaihautu wairewa.I roto i tenei tuhinga, ka tautuhia e matou te pūmua wiri wera pūtau 70 (Hsp70) ko te tauwehe kaihautu i whiriwhiria e hiahiatia ana mo te whakahohenga o te TBSV RdRp recombinant N-mutu kua tapahia.I tua atu, ko te aukati-iti-iti o te mahi Hsp70 ka aukati i te whakahiato RNA na te tombusvirus RdRp in vitro.I tua atu, ka whakaatuhia e matou ko nga phospholipids kūpapa, ara, phosphatidylethanolamine (PE) me te phosphatidylcholine (PC), ka whakarei ake i te RdRp whakahohe i roto i te vitro.He rereke, ko te phosphatidylglycerol (PG) e whakaatu ana i te kaha me te tino kaha ki te aukati i te whakahohenga RdRp in vitro.Ka whakaatuhia ano e matou ko te PE me te PC e whakaohooho ana i te RdRp-viral plus-strand RNA [(+)RNA] taunekeneke, i te mea ka aukati te PG i te herenga o te RNA viral ki te RdRp.I runga i nga mahi whakaihiihi me te aukati o nga momo phospholipids i roto i te tombusvirus RdRp whakahohenga, e kii ana matou ko te hanganga lipid o nga kiriuhi subcellular e whaaia ana ka whakamahia e nga tombusviruses ki te whakahaere i te huihuinga VRC hou i te wa o te mate. (RdRp), kei a ia te kawenga mo te whakahiato i te uri RNA viral i roto i nga pūtau pangia o te maha o nga wheori RNA pai-pai, karekau i te mahi i te tuatahi.Ko tenei rautaki ka karo i te whakahiatotanga RNA viral i roto i te cytosol ka tere ki te whakauru i nga whakautu pareketo pūtau i te RNA.I te wa o te huihuinga o te matatini taapiri-a-mepiu, ka whakahohehia te RdRp viral na roto i te tikanga kaore i tino maarama e taea ai e te RdRp te whakahiato RNA.Ma te whakamahi i te TBSV RdRp, e whakaatu ana matou ko te Hsp70 chaperone pūkoro me nga phospholipids kupapa e whakahaere ana i te whakahohe RdRp in vitro.He rereke, ko te phosphatidylglycerol (PG) he mana aukati i runga i te in vitro RdRp whakahohe me te RdRp-viral RNA taunekeneke, e kii ana ko te microdomain membranous e karapoti ana i te RdRp ka tino pa ki tona kaha mo te whakahiato RNA.No reira, ko te whakahohenga o te RdRp viral ka whakawhirinaki ki nga waahanga kaihautu maha i roto i nga pūtau kua pangia.