L-Proline Cas: 147-85-3 99% White paura
| Nama Putumōhio | XD90293 |
| Ingoa Hua | L-Proline |
| CAS | 147-85-3 |
| Tātai rāpoi | C5H9NO2 |
| Taumaha rāpoi | 115.13046 |
| Nga Taipitopito Rokiroki | Ambient |
| Waehere Rere Whakariterite | 29339980 |
Whakatakotoranga Hua
| Te whakamatautau | 99% min |
| Te ahua | Te paura ma |
| Te hurihanga motuhake | -84.5 ki -86 |
| Nga konganuku taumaha | <15ppm |
| AS | <1ppm |
| Ph | 5.9 - 6.9 |
| SO4 | <0.050% |
| Fe | <30ppm |
| Ngaronga i te whakamaroke | <0.3% |
| Te toenga i runga i te mura | <0.10% |
| NH4 | <0.02% |
| Cl | <0.050% |
| State of Solution | >98% |
He mea nui te mohio ki te paohotanga o te ope microbial mo te whanaketanga me te arotautanga o nga tikanga biocatalytic e ahu mai ana i nga pūtau-katoa, na te mea e tohu ana i te pai o te whakaputa.He tino tika tenei mo te redox biocatalysis e whakamahia ana nga pūtau hohe metabolic na te kaha o te cofactor/cosubstrate regenerative i roto i te ope.I whakamahia te Recombinant Escherichia coli mo te whakaputa nui o te proline-4-hydroxylase (P4H), he dioxygenase e whakakorikori ana i te hydroxylation o te L-proline kore utu ki te trans-4-hydroxy-L-proline me te a-ketoglutarate (a-KG) hei cosubstrate.I roto i tenei biocatalyst pūtau-katoa, ka whakaratohia e te waro waro o te pokapū te cosubstrate a-KG e hiahiatia ana, e hono ana i te mahi biocatalytic P4H ki te mahi waro me te mahi metabolic.Na roto i te whakamahi i nga taputapu koiora whakamatautau me te rorohiko, penei i te miihini miihini me te (13)C-metabolic flux analysis ((13)C-MFA), i tirotirohia e matou me te whakaahua ine ine te urupare a-tinana, metabolic, me te bioenergetic o te biocatalyst pūtau katoa. ki te hurihanga koiora kua whaaia me te tautuhi i nga kohungahunga metabolic pea mo te mahi miihini ara tika. I hangaia he riaka E. coli ngoikore-proline ma te whakakore i te ira putA whakawaehere proline dehydrogenase.Ko nga huringa koiora-a-katoa me tenei riaka mutant i arahi ki te whakamaarama proline ine engari ki te rearua o te reiti whakangao trans-4-L-hydroxyproline (hyp) motuhake, ka whakaritea ki te momo mohoao.Ko te tātaritanga o te rewharewha waro na roto i te whakahiatotanga o te riaka mutant i kitea ko te pikinga o te tono a-KG mo te mahi P4H kare i whakaniko i te rerenga whakaputa a-KG, e tohu ana i te whakahaerenga huringa TCA i raro i nga tikanga i akohia.I roto i te riaka momo mohoao, ko te whakahiato P4H me te catalysis na te hekenga o te hua koiora.Ko te mea whakamiharo, ko te riaka ΔputA i utu ano te mate ATP me te NADH e pa ana ma te whakaiti i nga tono hiko tiaki ki te iti o te reiti tango huka, hei utu mo te whakanui ake i te mahi TCA. I kitea te patoto putA i roto i te recombinant E. coli BL21(DE3)(pLysS) kia oati mo te P4H catalysis whai hua ehara i te mea anake mo te hua o te huringa koiora, engari mo nga reiti mo te huringa koiora me te tangohanga proline me te hua o te hyp i runga i te puna hiko.Ko nga hua e tohu ana, i runga i te tukituki putA, ko te hono o te TCA-huringa ki te proline hydroxylation ma te cosubstrate a-KG ka noho hei take matua e aukati ana me te whainga ki te whakapai ake i te pai o nga huringa koiora-a-KG.
