Hua

Ko te huringa koiora o te methyl-β-D-glucopyranoside ki te teitei o nga mekameka alkyl glucosides na roto i te β-glucosidase herea o Pichia etchellsii.

I roto i te rangahau o naianei, kua tirotirohia e matou te whakamahinga o nga pūtau katoa o Pichia etchellsii mo te whakahiato o nga glucosides alkyl mekameka roa.I whakamahia te Methyl-β-d-glucopyranoside (MG) hei tauhohenga me nga waipiro momona, n-hexanol, n-octanol, n-decanol me n-dodecanol ki te whakahiato i nga glucosides alkyl.Ko nga tikanga tauhohenga tuatahi i arotau tuatahi ki te tauine 2.5 ml mo te whakahiato o te octyl glucoside (OG) me te 8% te ihirangi wai, te 100mM MG me te 6h o te wa tauhohenga ka puta mai he ≈ 53% te hua.He 2.79 te nui o te transglucosylation/hydrolysis ōwehenga i whiwhi i te 100mM MG e pai ana ki te whakaputa hua nui.I runga i nga tikanga kua arotauhia, i whakahaeretia he reactor i te taumata 50 ml i hua mai i te ≈ 60% te hurihanga o MG ki OG.I hangaia he tikanga chromatography wai mahi nui mo te inenga o nga glucosides mekameka teitei ma te whakamahi i te kaitao tohu tohu.Ko te nui o te 27% me te 13% te hua i whiwhi mo te decyl-, me te dodecyl-β-d-glucopyranoside, ia.