Hua

I roto i te tikanga kinetic angiotensin-converting enzyme (ACE), he parepare whaihua me te tino pai ko te 80 mmol/L te parepare borate i te pH 8.2 (37 nga nekehanga C).Ka kitea he wa takamuri i roto i te tauhohenga, a ka whakaarohia he 5-meneti o te whakauru o te tïpako me te plasma i mua i te inenga.Ko te tïpako, N-[3-(2-furyl)acryloyl]-L-phenylalanylglycylglycine (FAPGG), ka whakanuia te kukū ki te 1.0 mmol/L me te roangaru ine kei te 345 nm hei whakarite i te rarangi o te ine.Ko te mahinga e whakaarohia ana e whakamahi ana i te 1:9 plasma-ki-reagent volume ratio.Ko te awhe raina o te whakamatautau ka toro atu ki te 170 U/L, e tohu ana i te 25% te hauwai o te tïpako.Ko te whakaurunga FAPGG ka whakatauhia ma te ine i te rereketanga o te whakaurunga i waenga i te 1.0 mmol/L FAPGG me nga otinga hua.Ka tirohia te pono o te roangaru ma te tohu i te uara whakaurunga e tumanakohia ana o te otinga FAPGG, a, ko te 1.0-nm te rereke mai i te 345 nm ka whakarereke i te whakaurunga ma te 15.5%.Ko te tika o nga whakamatautau ACE i te 60 me te 100 U/L he 3.5% me te 2.4% i roto i te puranga me te 2.9% me te 2.6% i waenga i te puranga, ia.Ko te waahi tohutoro (2.5th ki te 97.5th percentiles) he 41-139 U/L, a kaore he rereketanga i waenga i nga uara mo te tane me te wahine.