PIPES Cas: 5625-37-6 Te paura tioata ma 99% ABTS DIAMMONIUM SALT ULTRA PURE GRADE
| Nama Putumōhio | XD90117 |
| Ingoa Hua | PIPE (Piperazine-1,4-bis(2-ethanesulfonic acid)) |
| CAS | 5625-37-6 |
| Tātai rāpoi | C8H18N2O6S2 |
| Taumaha rāpoi | 302.37 |
| Nga Taipitopito Rokiroki | Ambient |
| Waehere Rere Whakariterite | 2933599 |
Whakatakotoranga Hua
| Nga konganuku taumaha | <5ppm |
| Ngaronga i te whakamaroke | <1.0% |
| Wairewa | Maama, Wairewa Taekore (5% 1N NaOH) |
| Te whakamatautau | 99 - 101% |
| Te ahua | Te paura tioata ma |
Ko nga paipa [piperazine-N,N′-bis(2-ethanesulfonic acid)] ka whakamahia i te nuinga o te wa hei maatete mo te matū koiora.He parepare waikawa ethanesulfonika i hangaia e Good et al.i nga tau 1960.He pKa te PIPES e tata ana ki te pH ahupūngao e whai hua ai i roto i nga mahi ahurea pūtau.Kua tuhia ki te whakaiti i te mate o te lipid i te wa e tarai ana te glutaraldehyde histology i roto i nga tipu me nga kopa kararehe.He kaihoko whakaahuru me te pKa tata ki te pH tinana.
Ko te antimicrobial peptide magainin 2 ka hanga pores i roto i nga kiriuhi lipid me te akiaki i te urunga o te membrane o nga ihirangi pūkoro.Ahakoa ko tenei urunga te take matua o tana mahi huakita, kare tonu i te maarama te tikanga o te hanga pore.Na reira i tirotirohia e maatau te taunekeneke o te magainin 2 me nga kiriuhi lipid ma te whakamahi i nga vesicles unilamellar nui kotahi (GUV).Ko te herenga o te magainin 2 ki te membrane lipid o nga GUV i whakanui ake i te huringa hautanga o te waahi o te membrane, δ, i rite ki te kukū o te mata o te magainin 2, X. Ko tenei e tohu ana ko te tere tere o te magainin 2-induced e rua. -te whakawhitinga o te ahua mai i te ahua mau ki te ahua pore kua tino piki ake me te pikinga o te δ.Ko te taumahatanga o te kiriuhi lipid i muri mai i te wawata o te GUV i whakahohea ano te hanga pore-2-induced magainin.Hei whakaatu i te waahi o te magainin 2, i tirotirohia te taunekeneke o te carboxyfluorescein (CF) -labeled magainin 2 (CF-magainin 2) me nga GUV kotahi kei roto he rangahau fluorescent wairewa-wai, AF647, i tirotirohia ma te whakamahi i te microscopy confocal.I te kore o te awangawanga na te wawata, i muri i te taunekeneke o te magainin 2 ko te kaha o te fluorescence o te rim GUV na te CF-magainin 2 ka piki tere ki te uara pumau, i noho tonu mo te wa roa, me te 4-32 s i mua. i te timatanga o te rerenga o te AF647 i timata te kaha o te rii ki te piki tere ki tetahi atu uara pumau.He rereke, i mua i te awangawanga, karekau he pikinga o te kaha o te rii i mua tonu i te tiimata o te rerenga i kitea.Ko enei hua e tohu ana kaore e taea e te magainin 2 te whakawhiti mai i waho ki te monolayer o roto tae noa ki mua noa i te hanganga pore.I runga i enei hua, ka whakatauhia e matou ko te maanin 2-induced pore he pore totoro-whakahohe me te toronga o te monolayer o roto ko te kaha taraiwa matua o te hanganga pore.
