He ira beta-glucosidase (bgl3) mai i Streptomyces sp.Ko te QM-B814 (American Type Culture Collection 11238) kua katihia e te taapiri mahi o te mutant beta-glucosidase-kino o Streptomyces lividans.He anga panui-tuwhera o te 1440 nucleotides e whakawae ana i te polypeptide o te 479 waikawa amino i kitea ma te raupapa.Ko te pūmua whakawaehere (Bgl3) e whakaatu ana i te tino rite (neke atu i te 45% te tuakiri) me te beta-glycosidases mai i te whanau-1 glycosyl hydrolases.Ko te whākōkī kakato, kua purea i muri i te rerenga haukinia pūhākawa me ngā kaupae chromatographic e rua, he monomeric me te papatipu rāpoi 52.6 kDa, e whakatauhia ana e te spectrometry papatipu, me te ira isoelectric o pI 4.4.Ko te ahua he beta-glucosidase me te whanui motuhake o te tïpako, he kaha ki te cellooligomers, me te mahi i nga tauhohenga transglycosylation.Ko nga uara Km kitea mo te p-nitrophenyl-beta-D-glucopyranoside me te cellobiose he 0.27 mM me te 7.9 mM.Ko nga uara Ki mo te hukahuka me te delta-gluconolactone, ma te whakamahi i te p-nitrophenyl-beta-D-glucopyranoside hei taputapu, he 65 mM me te 0.08 mM, ia.He pH tino pai o te enzyme purea ki te pH 6.5 me te pāmahana tino pai mo te mahi he 50 nga nekehanga
