He ira β-glucosidase pūrākau, bgl1G5, i kanohia mai i te Phialophora sp.G5 me te whakaatu angitu i roto i te Pichia pastoris.I tohuhia te tātari raupapa ko te ira he anga panui tuwhera 1,431-bp te whakawaehere i te pūmua o te 476 waikawa amino.I whakaatuhia e te raupapa waikawa amino o bgl1G5 he tuakiri teitei o te 85 % me te tohu β-glucosidase mai i Humicola grisea o te whanau glycoside hydrolase 1. Ka whakatauritea ki etahi atu harore, Bgl1G5 i whakaatu rite te mahi tino pai i te pH 6.0 me te 50 °C me te pumau. i te pH 5.0–9.0.I tua atu, i whakaatu a Bgl1G5 i te pai o te thermostability i te 50 °C (6 h te haurua o te ora) me nga mahi motuhake teitei ake (54.9 U mg-1).Ko nga uara K m me V max ki te p-nitrophenyl β-D-glucopyranoside (pNPG) he 0.33 mM me te 103.1 μmol min-1 mg-1, ia.Ko te whakamatautau motuhake o te substrate i whakaatu ko te Bgl1G5 he tino kaha ki te pNPG, he ngoikore i runga i te p-nitrophenyl β-D-cellobioside (pNPC) me te p-nitrophenyl-β-D-galactopyranoside (ONPG), kaore he mahi i runga i te cellobiose.
