Hua

Ko te Bicine he waikawa amino zwitterionic, he kaha i roto i te awhe pH 7.6-9.0 (pKa o 8.26 i te 25°C).He parepare e taunaki ana mo te mahi matū koiora iti.Ka whakamahia te Bicine mo te whakarite i te otinga tïpako pumau mo te whakatau i te serum guanase.Ko te whakamahinga o te paihikara i roto i te tikanga chromatography whakawhiti katote angiangi mo te whakatau pūmua kua whakaputaina.Kua whakamahia te Bicine ki te peptide me te whakatioatanga pūmua.He rangahau whakanekeneke o te huinga tauwhitinga-a-whaa-taiao o te creatine kinase i whakamahia te paihikara i roto i te parepare tauhohenga.Kua whakaahuahia he punaha parepare maha mo te SDS-PAGE o nga pūmua me nga peptides e whakauru ana i te paihikara.

Ko nga pakitara alveolar kotahi i raro i nga rangahau-roa-roa i roto i te saline me te Bicine (0.2 M) ka paheke haere i roto i te ahotea kiko (TTD).Kua tirohia e matou te paanga o nga otinga rereke mo tenei TTD me te rapu mo nga huringa e rite ana ki te ultrastructure.I wehea te parenchyma o te pukahukahu ki nga pakitara pukoro kotahi (30 X 30 X 150 microns) i roto i te saline phosphate-buffered (0.15 M).Ka whakawhitia ki te pati kaukau roa, ka rukuhia te kiko ki te Bicine, te tote, te wairewa a Hank whakakaha, 0.25% Alcian puru i roto i te tote, he otinga konutai dodecyl sulfate ranei, mo nga wa rereke.I hurihia i roto i tetahi toronga kua tohua me te kaha teitei i inehia i roto i te waa, ko enei kopa ano i mau ki roto i te glutaraldehyde/waikawa tannic me te tukatuka mo te miihini irahiko.Ko nga pakitara pukoro kotahi i rumakina ki te tote, ki te Bicine ranei ka whakaatu he TTD ahu whakamua.I puta mai he mokowai, mokowhiti ranei i roto i te interstitium i ahu whakamua me te whakararurarutanga pūkoro me te TTD.Ka kitea i roto i te 0.3 haora, he pai te ahu whakamua o nga huringa i te 0.6 haora.I roto i te konutai dodecyl sulfate (70 mM), heoi, karekau he TTD me te hanganga karekau he interstitium, ko nga kiriuhi o raro anake me nga pūmua kiriu e toe ana.I roto i te wairewa a Hank whakakaha, i te 0.25% Alcian kahurangi ranei, i tiakina paitia te matrix interstitial, te morphology pūtau me te ahotea o te kiko mo te 1 haora.E ai ki tenei rangahau ka puta te rewharewha o te kopu interstitial i roto i te tote, i te Bicine ranei, a he mea nui te matrix mau tonu mo te pupuri i te taumahatanga o te kiko.