dCMP, 2'-Deoxycytidine 5'-monophosphate, waikawa kore utu CAS:1032-65-1
| Nama Putumōhio | XD90549 |
| Ingoa Hua | dCMP, 2'-Deoxycytidine 5'-monophosphate, waikawa kore utu |
| CAS | 1032-65-1 |
| Tātai rāpoi | C9H14N3O7P |
| Taumaha rāpoi | 307.20 |
| Nga Taipitopito Rokiroki | -15 ki te -20 °C |
| Waehere Rere Whakariterite | 29349990 |
Whakatakotoranga Hua
| Te ahua | Mārō |
| Te whakamatautau | 99% |
Ko te Beta-D-2'-deoxy-2'-fluoro-2'-C-methylcytidine (PSI-6130) he kaha te aukati motuhake o te whakahiatotanga RNA kakā C huaketo (HCV) RNA i roto i nga pūtau tauira Huh-7.Hei aukati i te HCV NS5B RNA polymerase, me phosphorylated te PSI-6130 ki te ahua 5'-triphosphate.I tirotirohia te phosphorylation o PSI-6130 me te aukati i te HCV NS5B.Ko te phosphorylation o te PSI-6130 e te recombinant human 2'-deoxycytidine kinase (dCK) me te uridine-cytidine kinase 1 (UCK-1) i inehia ma te whakamahi i te tauhohenga spectrophotometric hono.I whakaatuhia ko PSI-6130 he taputapu mo te dCK kua purea, me te Km o 81 microM me te kcat o 0.007 s-1, engari ehara i te taputapu mo te UCK-1.Ko te PSI-6130 monophosphate (PSI-6130-MP) he pai te phosphorylated ki te diphosphate me muri ki te triphosphate na te recombinant tangata UMP-CMP kinase me te nucleoside diphosphate kinase, ia.I akohia te aukati o te momo mohoao me te whakarereke (S282T) HCV NS5B RNA polymerases.Ko te u tonu te inhibition inhibition (Ki) mo te PSI-61 30 triphosphate (PSI-6130-TP) me te enzyme mohoao-momo ko te 4.3 microM.He rite nga hua ki te 2'-C-methyladenosine triphosphate (Ki=1.5 microM) me te 2'-C-methylcytidine triphosphate (Ki=1.6 microM).Ko te NS5B me te whakarereketanga S282T, e mohiotia ana ki te tuku parenga ki te 2'-C-methyladenosine, i aukatihia e te PSI-6130-TP kia rite ki te momo mohoao.Ko te whakaurunga o te PSI-6130-MP ki roto i te RNA i whakakorikoria e te NS5B RNA polymerase kua purea ka mutu te mekameka.
