Hua

Monogalactopyranosides o te fluorescein me te fluorescein methyl ester: te whakahiatotanga, te hauwai enzymatic na roto i te biotnylated β-galactosidase, me te whakatau i te whakarea whakamaoritanga whakamaoritanga

Ko nga monoglycosides Fluorescein (d-galactopyranoside (FMG) me te d-glucopyranoside) me to ratou methyl ester (MFMG) kua whakaritea mai i te acetobromoglucose/galactose me te fluorescein methyl ester i roto i nga hua pai.Kua mahia nga whakamatautau hauwai hau (ma te whakamahi i te β-galactosidase biotinylated) o nga pärönaki galacto me te tatau i nga tawhā neke.Kua kitea he 15-20 nga wa te pikinga o te kaha o te rewharewha i te wa o te hauwai.Kua kitea te pikinga o te rewharewha i te wa poto me te iti o te kukū o te tïpako, na te mea ka whai hua enei puhui me nga rangahau tairongo mo nga galactosidases.Ko te nui o te uara taumau Michaelis–Menten (Km) mo te MFMG he teitei ake i tera o te FMG e tohu ana ka taea te whakarereketanga o te taputapu fluorogenic.He iti ake te uara Km mo te β-Gal biotinylated me te FMG i tera mo te enzyme taketake.E tohu ana tenei tirohanga i te hononga o te tïpako nui ake o te enzyme biotinylated ki te whakataurite ki te enzyme taketake.Kua inehia nga whakarea whakamaoritanga whakamaoritanga, mo nga tïpako fluorogenic me nga hua e rua, e whakamahi ana i te hihinga whakahiatotanga o te rere.Ko nga whakarea whakamaoritanga whakamaoritanga mo nga taputapu fluorogenic me nga hua hauwai hauwai kua inehia kia rite, i roto i te awhe 3.5–4.5 × 10−10 m2 s−1.No reira karekau e tino kitea te whakanikotanga, te whakaroa ranei o nga kinetika enzymatic na te rerekeetanga o te nekeneke whakamaori o te tïpako me te hua.